Appendix I - SUPPLEMENT - Number 4
TITLE: Special inspection requirements regarding Spring Viremia of Carp (SVC, Rhabdovirus carpio)
INTRODUCTION: Spring Viremia of Carp, caused by the agent Rhabdovirus carpio, is in the
Prohibited Disease/Pathogen category.
Inspection procedures for this disease and the agent causing it are included in the Inspection Regimen listed below. However, since this disease is currently not known to occur in North America, inspection for this disease/pathogen is only required for importation of non-salmonid fishes from outside of North America. Inspecting agents shall evaluate inspections completed by source located fish health specialists.
Clinical signs include:
1) dark body color;
2) loss of equilibrium;
3) exophthalmia;
4) abdominal distention;
5) inflamed and edematous vent;
6) peritonitis;
7) ascites;
8) catarrhal enteritis; and
9) petechiae in heart, liver, kidneys, intestines, internal wall of swimbladder and skeletal muscle.
INSPECTION REGIMEN:
1. Assume no greater than 5% carrier prevalence and sample for 95% confidence.
2. Sample processing:
a) Use standard inoculum preparation procedures employing whole fry, viscera, or kidney/spleen/liver samples.
b) Employ FHM, EPC, RTG-2, BB, GHK-21, chick embryo fibroblasts or other cell lines demonstrated to be sensitive to Rhabdovirus carpio.
c) Incubate primary inoculation assays for seven (7) days in 20°-22°C temperature range.
3. Virus identification:
a) Positively identify serologically, if possible.
b) Presumptively identify by characterization of CPE and inability of other antiseras to neutralize infectivity (if specific antiserum is not available).