Section 179-25-007 - ANALYTICAL METHODS007.01 Cryptosporidium Systems must analyze for Cryptosporidium using Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA, 2005, United States Environmental Protection Agency, EPA-815-R- 05-002 or Method 1622: Cryptosporidium in Water by Filtration/IMS/FA, 2005, United States Environmental Protection Agency, EPA-815-R- 05-001, which are incorporated by reference or an equivalent method approved by EPA. A copy of these methods may be obtained online from http://www.epa.gov/safewater/disinfection/lt2 or from the United States Environmental Protection Agency, Office of Ground Water and Drinking Water, 1201 Constitution Ave., NW, Washington, DC 20460 (Telephone: 800-426-4791). A copy may be inspected at the office of the Division of Public Health of the Department of Health and Human Services, 301 Centennial Mall South, Lincoln, NE 68509.
25-007.01A Systems must analyze at least a 10 liter (L) sample or a packed pellet volume of at least 2 milliliter (mL) as generated by the methods listed in 179 NAC 25-007.01. Systems unable to process a 10 L sample must analyze as much sample volume as can be filtered by two filters approved by EPA for the methods listed in 179 NAC 25-007.01, up to a packed pellet volume of at least 2 mL.25-007.01B Procedure for Matrix Spike Samples25-007.01B1 Matrix Spike (MS) samples, as required by the methods in 179 NAC 25-007.01, must be spiked and filtered by a laboratory approved for Cryptosporidium analysis under 179 NAC 25-008.25-007.01B2 If the volume of the MS sample is greater than 10 L, the system may filter all but 10 L of the MS sample in the field, and ship the filtered sample and the remaining 10 L of source water to the laboratory. In this case, the laboratory must spike the remaining 10 L of water and filter it through the filter used to collect the balance of the sample in the field.25-007.01C Flow cytometer-counted spiking suspensions must be used for MS samples and ongoing precision and recovery (OPR) samples.007.02 E. coli Systems must use methods for enumeration of E. coli in source water approved in 40 CFR 136.3(a) or equivalent methods approved by EPA. Available from American Public Health Association, 800 I Street, NW, Washington, DC 20001-3710.
25-007.02A The time from sample collection to initiation of analysis may not exceed 30 hours unless the system meets the condition of 179 NAC 25-007.02B.25-007.02B The Department may approve on a case-by-case basis the holding of an E. coli sample for up to 48 hours between sample collection and initiation of analysis if the Department determines that analyzing an E. coli sample within 30 hours is not feasible. E. coli samples held between 30 to 48 hours must be analyzed by the Colilert reagent version of Standard Method 9223B as listed in 40 CFR 136.3(a).25-007.02C Systems must maintain samples between 0º C and 10º C during storage and transit to the laboratory.007.03 Turbidity Systems must use methods for turbidity measurement approved in 179 NAC 13-007.01A.
179 Neb. Admin. Code, ch. 25, § 007