Wis. Admin. Code Department of Natural Resources NR 809.323

Current through October 28, 2024

Section NR 809.323 - Analytical requirements for groundwater source microbiological contaminants

(1) ANALYTICAL METHODS.

(a) A water supplier for a groundwater system subject to the source water monitoring requirements of s. NR 809.325(2) shall collect a standard sample volume of at least 100 mL for E. coli analysis regardless of the analytical method used.

(b) A water supplier for a groundwater system shall analyze all groundwater source samples, collected under s. NR 809.325(2), using one of the analytical methods listed in Table G for the presence of E. coli. If the department requires a public water system to be tested for the presence of enterococci or coliphage, the water supplier shall use one of the methods listed in Table G.

(c) Sample volumes of at least 100 mL shall be used for all analyses using the methods in Table G. Analyses shall be conducted in accordance with the documents listed in the footnotes to Table G.

(d) The sample holding time shall not exceed 30 hours. In all cases, samples should be analyzed as soon after collection as possible.

Table G

Analytical Methods for Source Water Monitoring

Fecal indicator	Methodology	Method Citation
E. coli	Colilert 2	9223 B1
	Colisure 2	9223 B1
	Membrane Filter Method EPA Method with MI Agar.	16043
	m-ColiBlue24 Test 4 E*Colite Test 5 EC-MUG 6	9221 F1
	NA-MUG 6	9222 G1
Enterococci	Multiple-Tube Technique.	9230 B1
	Membrane Filter Technique.	9230 C1
	Membrane Filter Technique.	1600 7
	Enterolert 8
Coliphage	Two-Step Enrichment EPA Method Presence-Absence Procedure.	16019
	Single Agar Layer Procedure.	160210

Note: Copies of the documents listed in the footnotes may be obtained from the sources listed in the footnotes. Copies may be inspected at EPA's Drinking Water Docket, EPA West, 1301 Constitution Avenue, NW., Room B102, Washington DC 20460, Telephone: 202-566-2426, or at the National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call 202-741-6030, or go to: http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html.

1 Methods are described in Standard Methods for the Examination of Water and Wastewater 20th edition (1998) and copies may be obtained from the American Public Health Association, 1015 Fifteenth Street, NW., Washington, DC 20005-2605.

2 Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092.

3 EPA Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium); September 2002, EPA 821-R-02-024. Method is available at http://www.epa.gov/microbes/documents/1604sp02.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

4 A description of the m-ColiBlue24 Test, "Total Coliforms and E. coli Membrane Filtration Method with m-ColiBlue24 Broth," Method No. 10029 Revision 2, August 17, 1999, is available from Hach Company, 100 Dayton Ave., Ames, IA 50010 or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

5 A description of the E*Colite Test, "Charm E*Colite Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Drinking Water," January 9, 1998, is available from Charm Sciences, Inc., 659 Andover St., Lawrence, MA 01843-1032 or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

6 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E. coli testing step as described in Sec. 141.21(f)(6)(i) or (ii) after use of Standard Methods 9221 B, 9221 D, 9222 B, or 9222 C.

7 EPA Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-[beta]-D-Glucoside Agar (mEI) EPA 821-R- 02-022 (September 2002) is an approved variation of Standard Method 9230C. The method is available at http://www.epa.gov/microbes/documents/1600sp02.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

8 Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092. Preparation and use of the medium is set forth in the article "Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters," by Budnick, G.E., Howard, R.T., and Mayo, D.R., 1996, Applied and Environmental Microbiology, 62:3881-3884.

9 EPA Method 1601: Male-specific (F+) and Somatic Coliphage in Water by Two-step Enrichment Procedure; April 2001, EPA 821-R-01-030. Method is available at http://www.epa.gov/microbes/documents/1601ap01.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

10 EPA Method 1602: Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure; April 2001, EPA 821-R-01-029. Method is available at http://www.epa.gov/microbes/documents/1602ap01.pdf or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

(2) INVALIDATION OF AN E. coli -positive groundwater source sample.

(a) The department may invalidate an E. coli-positive groundwater source sample collected under s. NR 809.325(2) under the conditions specified in subd. 1. or 2.

1. The water supplier provides the department with written notice from the laboratory that improper sample analysis occurred.

2. The department determines and documents in writing that there is substantial evidence that an E. coli-positive groundwater source sample is not related to source water quality.

(b) If the department invalidates an E. coli-positive groundwater source sample, the water supplier shall collect another source water sample under s. NR 809.325(2) no later than 24 hours after being notified by the department that the sample has been invalidated. The water supplier shall have the sample analyzed for E. coli using the analytical methods in s. NR 809.323(1) (b) Table G.

(c) The department may extend the 24-hour time limit on a case-by-case basis if the water supplier cannot collect the source water sample within the 24-hour time limit due to circumstances beyond the water supplier's control. In the case of an extension, the department shall specify in writing how much time the water supplier has to collect the sample.

Wis. Admin. Code Department of Natural Resources NR 809.323

CR 09-073: cr. Register November 2010 No. 659, eff. 12-1-10.

Amended by, CR 15-049: am. (1) (a), (b), (d) Table G, (2) (title), (a), (b) Register March 2016 No. 723, eff.4/1/2016