C.M.R. 01, 001, ch. 252, app 001-252-I

Current through 2024-51, December 18, 2024

Appendix 001-252-I - Laboratory procedures at an authorized Nuclear Seed Origination Source involved in producing Nuclear Seed[1]

In the Laboratory & Greenhouse

1. Disease Testing Tubers

a. Sprout tubers & remove apical tip

b. Plant remainder in greenhouse

c. Disease Test² and discard

d. Transfer tip to test tube culture medium and grow plantlet

e. Section plantlets into separate nodes,

f. Disease Test² one node 2. Produce plantlets

3. Transplant plantlets to greenhouse.

4. Harden plants off and Disease Test²

5. Grow to produce minitubers

Planted in soil (First Year) (See Section 2. B.)

1. Plant minitubers in field (Disease Test²)

2. Transplant plantlets into field (Disease Test²)

¹ Other methods of Pre-nuclear Seed production are acceptable. (See 2.C.2.)

² Laboratory test means testing for the Bacterial Ring Rot, Blackleg, PLRV, PVA, PVS, PVX, PVY, PVY-N, Potato Mop Top Virus, and PSTV pathogens.

C.M.R. 01, 001, ch. 252, app 001-252-I