* The department approves the use ofE. coli as the fecal indicator,
* The system only has one groundwater source required to be sampled,
* The system has no treatment, and
* Should the source water sample beE. coli -positive, the system would incur an acute coliform bacteria maximum contaminant level violation, must comply with Tier 1 public notification requirements, and must also comply with the additional sample monitoring in 41.7(3)"a" (3).
* A wholesale groundwater system that receives notice from a consecutive system it serves that a sample collected under 41.2(1)"f" through 41.2(1)"i" is total coliform-positive must, within 24 hours of being notified, collect triggered sample(s) from its groundwater source(s) under 41.7(3)"a" (2) and analyze the sample(s) for a fecal indicator.
* If the triggered source sample(s) is fecal indicator-positive, the wholesale groundwater system must notify all consecutive systems served by that groundwater source of the fecal indicator-positive result within 24 hours of being notified of the result and must collect the required additional five samples from the source within 24 hours under 41.7(3)"a" (3).
Analytical Methods for Source Water Monitoring
Fecal Indicator1 | Methodology | Method Citation |
E. coli | Colilert3 | 9223B2,12,13 9223 B-97, B-0418 |
Coli sure3 | 9223B2,12,13 9223B-97, B-0418 | |
Membrane filter method with MI agar | EPA Method 16044 | |
Colilert-18 | 9223B2,12,13 9223B-97, B-0418 | |
m-ColiBlue24 Test5 | ||
E*Colite Test6 | ||
EC-MUG7 | 9221F2,13 9221 F-0618 | |
NA-MUG7 | 9222G2 | |
Readycult | Readycult14 | |
Colitag | Modified Colitag15 | |
Chromocult | Chromocult16 | |
Tecta EC/TC | Tecta EC/TC 19 | |
Enterococci | Multiple-tube technique | 9230B29230 B-0418 |
Membrane filter technique | 9230C2 | |
Membrane filter technique | EPA Method 16008 | |
Enterolert9 | ||
Coliphage | Two-step enrichment presence-absence procedure | EPA Method 160110, FastPhage17 |
Single agar layer procedure | EPA Method 160211 |
Analyses must be conducted in accordance with the documents listed below. The Director of the Federal Register approves the incorporation by reference of the documents listed in footnotes 2 through 11 in accordance with 5 U.S.C. 552(a) and 1 CFR Part 51. Copies of the documents may be obtained from the sources listed below. Copies may be inspected at EPA's Drinking Water Docket, EPA West, 1301 Constitution Avenue, NW, EPA West Room B102, Washington, DC 20460; (telephone: (202)566-2426); or at the National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call (202)741-6030, or go to: www.archives.gov/federal_register/code_of_federal _regulations/ibr_locations.html .
The address for EPA's Water Resource Center, referenced in several of the footnotes, is EPA Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW, Washington, DC 20460.
1The time from sample collection to initiation of analysis may not exceed 30 hours. The groundwater system is encouraged but is not required to hold samples below 10°C during transit.
2Methods are described in Standard Methods for the Examination of Water and Wastewater, 20th edition (1998), and copies may be obtained from the American Public Health Association, 800 I Street, NW, Washington, DC 20001-3710.
3Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092.
4EPA Method 1604: Total Coliforms andEscherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium); September 2002, EPA 821-R-02-024. Method is available at www.nemi.gov.
5A description of the m-ColiBlue24 Test, "Total Coliforms andE. coli Membrane Filtration Method with m-ColiBlue24 Broth," Method No. 10029, Revision 2, August 17, 1999, is available from Hach Company, 100 Dayton Avenue, Ames, IA 50010.
6A description of the E*Colite Test, "Charm E*Colite Presence/Absence Test for Detection and Identification of Coliform Bacteria andEscherichia coli in Drinking Water," January 9, 1998, is available from Charm Sciences, Inc., 659 Andover Street, Lawrence, MA 01843-1032.
7EC-MUG (Method 922 IF) or NA-MUG (Method 9222G) can be used for E. coli testing step as described in 41.2(1)"f"(6) or (7) after use of Standard Method 9221B, 9221D, 9222B, or 9222C.
8EPA Method 1600: Enterococci in Water by Membrane Filtration Using Membrane-Enterococcus Indoxyl-p-D-Glucoside Agar (MEI), EPA 821-R-02-022 (September 2002), is an approved variation of Standard Method 9230C. The method is available at www.nemi.gov. The holding time and temperature for groundwater samples is specified in footnote 1 above, rather than as specified in Section 8 of EPA Method 1600.
9Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092. Preparation and use of the medium is set forth in the article "Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters" by Budnick, G.E., Howard, R.T., and Mayo, D.R., 1996, Applied and Environmental Microbiology, 62:3881-3884.
10EPA Method 1601: Male-Specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure; April 2001, EPA 821-R-01-030. Method is available at www.nemi.gov.
"EPA Method 1602: Male-Specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure; April 2001, EPA 821-R-01-029. Method is available at www.nemi.gov.
12Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from the American Public Health Association, 800 I Street, NW, Washington, DC 20001-3710.
13Standard Methods for the Examination of Water and Wastewater, 22nd edition (2012). Available from the American Public Health Association, 800 I Street, NW, Washington, DC 20001-3710.
14Readycult Method, "Readycult Coliforms 100 Presence/Absence Test for Detection and Identification of Coliform Bacteria andEscherichia coli in Finished Waters," January 2007, Version 1.1. Available from EMD Millipore, 290 Concord Road, Billerica, MA 01821.
15Modified Colitag Method, "Modified Colitag Test Method for the Simultaneous Detection of E.coli and Other Total Coliforms in Water (ATP D05-0035)," August 28, 2009. Available from www.nemi.gov or CPI International, 5580 Skylane Blvd., Santa Rosa, CA 95403.
16Chromocult Method, "Chromocult Coliform Agar Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria andEscherichia coli in Finished Waters," November 2000, Version 1.0. Available from EMD Millipore, 290 Concord Road, Billerica, MA 01821.
17Charm Sciences, Inc., "FastPhage Test Procedure. Presence/Absence for Coliphage in Ground Water with Same Day Positive Prediction," Version 009, November 2012. Available atwww.charmsciences.com.
18Standard Methods Online is available at www.standardmethods.orgThe year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.
19TectaEC/TC. "Presence/Absence Method for Simultaneous Detection of Total Coliforms andEscherichia coli in Drinking Water," April 2014. Available from Veolia Water Solutions and Technologies, Suite 4697, Biosciences Complex, 116 Barrie Street, Kingston, Ontario, Canada K7L 3N6.
* Any subsequent modifications to a department-approved corrective action plan and schedule must also be approved by the department; and
* If the department specifies interim measures for protection of the public health pending department approval of the corrective action plan and schedule, or pending completion of the corrective action plan, the system must comply with these interim measures as well as with any schedule specified by the department.
* The nature of the significant deficiency and the date the significant deficiency was identified by the department;
* The department-approved plan and schedule for correction of the significant deficiency, including interim measures, progress to date, and any interim measures completed; and
* For systems with a large proportion of non-English speaking consumers, as determined by the department, information in the applicable language(s) regarding the importance of the notice or a telephone number or address where consumers may contact the system to obtain a translated copy of the notice or assistance in the appropriate language.
* A groundwater system serving more than 3,300 people must continuously monitor the residual disinfectant concentration, using analytical methods specified in 567-subparagraph 43.5(4)"a "(5), at a location approved by the department and must record the lowest residual disinfectant concentration each day that water from the groundwater source is served to the public. The groundwater system must maintain the department-determined minimum residual disinfectant concentration every day the groundwater system serves water from the groundwater source to the public. If there is a failure in the continuous monitoring equipment, the groundwater system must conduct grab sampling every four hours until the continuous monitoring equipment is returned to service. The system must resume continuous residual disinfectant monitoring within 14 days.
* A groundwater system serving 3,300 or fewer people must monitor the residual disinfectant concentration using analytical methods specified in 567-subparagraph 43.5(4)"a"(5) at a location approved by the department and must record the residual disinfectant concentration each day that water from the groundwater source is served to the public. The groundwater system must maintain the department-determined minimum residual disinfectant concentration every day the groundwater system serves water from the groundwater source to the public. The groundwater system must take a daily grab sample during the hour of peak flow or at another time specified by the department. If any daily grab sample measurement falls below the department-determined minimum residual disinfectant concentration, the groundwater system must take follow-up samples every four hours until the residual disinfectant concentration is restored to the department-determined minimum level. Alternatively, a groundwater system that serves 3,300 or fewer people may monitor continuously and meet the requirements of 41.7(4)"b" (3)" 1," first bulleted paragraph.
* The membrane has an absolute molecular weight cut-off (MWCO), or an alternate parameter that describes the exclusion characteristics of the membrane, that can reliably achieve at least 4-log removal of viruses;
* The membrane process is operated in accordance with department-specified compliance requirements; and
* The integrity of the membrane is intact.
* Monitor the alternative treatment in accordance with all department-specified monitoring requirements; and
* Operate the alternative treatment in accordance with all compliance requirements that the department determines to be necessary to achieve at least 4-log treatment of viruses.
Iowa Admin. Code r. 567-41.7