A reverse mutation test in either Salmonella typhimurium or Escherichia coli detects mutation in an amino-acid requiring strain (histidine or tryptophan, respectively) to produce a strain independent of an outside supply of amino-acid.
Base pair substitution mutagens are agents that cause a base change in DNA. In a reversion test this change may occur at the site of the original mutation, or at a second site in the bacterial genome.
Frameshift mutagens are agents that cause the addition or deletion of one or more base pairs in the DNA, thus changing the reading frame in the RNA
Chemical | CAS No. |
9,10-Dimethylanthracene | [CAS no. 781-43-1] |
7,12-Dimethylbenzanthracene | [CAS no. 57-97-6] |
Congo Red (for the reductive metabolic activation method) | [CAS no. 573-58-0] |
Benzo(a)pyrene | [CAS no. 50-32-8] |
Cyclophosphamide (monohydrate) | [CAS no. 50-18-0] [CAS no. 6055-19-2] |
2-Aminoanthracene | [CAS no. 613-13-8] |
Chemical | CAS No. | Strain |
(a) Sodium azide | [CAS no. 26628-22-8] | TA1535 and TA100 |
(b) 2-Nitrofluorene | [CAS no. 607-57-8] | TA 98 |
(c) 9-Aminoacridine or ICR 191 | [CAS no. 90-45-9] or [CAS no. 17070-45-0] | TA1537, TA97 and TA97a |
(d) Cumene hydroperoxide | [CAS no. 80-15-9] | TA102 |
(e) Mitomycin C | [CAS no. 50-07-7] | WP2 uvrA and TA102 |
(f) N-Ethyl-N-nitro-N-nitrosoguanidine or 4-nitroquinoline 1-oxide | [CAS no. 70-25-7] or [CAS no. 56-57-5] | WP2, WP2 uvrA and WP2 uvrA (pKM101) |
(g) Furylfuramide (AF-2) | [CAS no. 3688-53-7] | Plasmid-containing strains |
40 C.F.R. §799.9510