Table-Biochemical Pesticides Human Health Assessment Data Requirements
Guideline Number | Data Requirement | Use Patterns | Test Substance | Test Notes | ||
Food | Nonfood | MP | EP | |||
Tier I | ||||||
Acute Testing | ||||||
870.1100 | Acute oral toxicity - rat | R | R | TGAI and MP | TGAI and EP | 1 |
870.1200 | Acute dermal toxicity | R | R | TGAI and MP | TGAI and EP | 1, 2 |
870.1300 | Acute inhalation toxicity - rat | R | R | TGAI and MP | TGAI and EP | 3 |
870.2400 | Primary eye irritation - rabbit | R | R | TGAI and MP | TGAI and EP | 2 |
870.2500 | Primary dermal irritation | R | R | TGAI and MP | TGAI and EP | 1, 2 |
870.2600 | Dermal sensitization | R | R | TGAI and MP | TGAI and EP | 2, 4 |
none | Hypersensitivity incidents | R | R | All | All | 5 |
Subchronic Testing | ||||||
870.3100 | 90-day oral (one species) | R | CR | TGAI | TGAI | 6 |
870.3250 | 90-day dermal - rat | CR | CR | TGAI | TGAI | 7 |
870.3465 | 90-day inhalation - rat | CR | CR | TGAI | TGAI | 8 |
Developmental Toxicity | ||||||
870.3700 | Prenatal developmental - rat preferably | R | CR | TGAI | TGAI | 9 |
Mutagenicity Testing | ||||||
870.5100 | Bacterial reverse mutation test | R | CR | TGAI | TGAI | 10 |
870.5300 870.5375 | In vitro mammalian cell assay | R | CR | TGAI | TGAI | 10, 11 |
Tier II | ||||||
Mutagenicity Testing (In vivo cytogenetics) | ||||||
870.5385 870.5895 | In vivo Mammalian Cytogenetics | CR | CR | TGAI | TGAI | 13 |
Developmental Toxicity | ||||||
870.3700 | Prenatal developmental | CR | CR | TGAI | TGAI | 9 |
Special Tests | ||||||
880.3550 | Immunotoxicity | CR | CR | TGAI | TGAI | 12, 13 |
Applicator/User Exposure | ||||||
875.1100 | Dermal outdoor exposure | CR | CR | TGAI | TGAI | 15 |
875.1200 | Dermal indoor exposure | CR | CR | TGAI | TGAI | 15 |
875.1300 | Inhalation outdoor exposure | CR | CR | TGAI | TGAI | 15 |
875.1400 | Inhalation indoor exposure | CR | CR | TGAI | TGAI | 15 |
875.1500 | Biological monitoring | CR | CR | TGAI | TGAI | 15 |
Tier III | ||||||
Chronic Testing/Special Testing | ||||||
880.3800 | Immune response | CR | CR | TGAI | TGAI | 14 |
870.3800 | Reproduction and fertility effects | CR | CR | TGAI | TGAI | 16 |
870.4100 | Chronic oral - rodent and nonrodent | CR | CR | TGAI | TGAI | 17 |
870.4200 | Carcinogenicity - two species - rat and mouse preferred | CR | CR | TGAI | TGAI | 18 |
870.5380 | Mammalian spermatogonial chromosome aberration test | CR | CR | TGAI | TGAI | 19 |
Special Testing | ||||||
870.7200 | Companion animal safety | CR | CR | NR | TGAI or EP | 20 |
The use of a combined study that utilizes the two-generation reproduction study in rodents (guideline 870.3800) as a basic protocol for the addition of other endpoints or functional assessments in the immature animal is encouraged.
In addition, a 90-day range finding study in both rats and mice is required to determine the dose levels if carcinogenicity studies are required. If the mouse carcinogenicity study is not required, the 90-day mouse subchronic study is likewise not required.
40 C.F.R. §158.2050