Organism | Methodology category | Method1 | Citation1 |
Total Coliforms | |||
Lactose Fermentation Methods | Standard Total Coliform Fermentation Technique | Standard Methods 9221 B.1, B.2 (20th ed.; 21st ed.).2 3 | |
Standard Methods Online 9221 B.1, B.2-99.2 3 | |||
Presence-Absence (P-A) Coliform Test | Standard Methods 9221 D.1, D.2 (20th ed.; 21st ed.).2 7 | ||
Standard Methods Online 9221 D.1, D.2-99.2 7 | |||
Membrane Filtration Methods | Standard Total Coliform Membrane Filter Procedure | Standard Methods 9222 B, C (20th ed.; 21st ed.).2 4 | |
Standard Methods Online 9222 B-972 4, 9222 C-97.2 4 | |||
Membrane Filtration using MI medium | EPA Method 1604.2 | ||
m-ColiBlue24® Test2 4 | |||
Chromocult2 4 | |||
Enzyme Substrate Methods | Colilert® | Standard Methods 9223 B (20th ed.; 21st ed.).2 5 | |
Standard Methods Online 9223 B-97.2 5 | |||
Colisure® | Standard Methods 9223 B (20th ed.; 21st ed.).2 5 6 | ||
Standard Methods Online 9223 B-97.2 5 6 | |||
E*Colite® Test2 | |||
Readycult® Test2 | |||
modified Colitag® Test2 | |||
Escherichia coli | |||
Escherichia coli Procedure (following Lactose Fermentation Methods) | EC-MUG medium | Standard Methods 9221 F.1 (20th ed.; 21st ed.)2 | |
Escherichia coli Partition Method | EC broth with MUG (EC-MUG) | Standard Methods 9222 G.1c(2) (20th ed.; 21st ed.)2 8 | |
NA-MUG medium | Standard Methods 9222 G.1c(1) (20th ed.; 21st ed.)2 | ||
Membrane Filtration Methods | Membrane Filtration using MI medium m-ColiBlue24® Test2 4 | EPA Method 16042 | |
Chromocult2 4 | |||
Enzyme Substrate Methods | Colilert® | Standard Methods 9223 B (20th ed.; 21st ed.)2 5 | |
Standard Methods Online 9223 B-972 5 6 | |||
Colisure® | Standard Methods 9223 B (20th ed.; 21st ed.)2 5 6 | ||
Standard Methods Online 9223 B-972 5 6 | |||
E*Colite® Test2 | |||
Readycult® Test2 | |||
modified Colitag® Test2 |
1 The procedures must be done in accordance with the documents listed in paragraph (c) of this section. For Standard Methods, either editions, 20th (1998) or 21st (2005), may be used. For the Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits following the hyphen in the method number. The methods listed are the only online versions that may be used. For vendor methods, the date of the method listed in paragraph (c) of this section is the date/version of the approved method. The methods listed are the only versions that may be used for compliance with this rule. Laboratories should be careful to use only the approved versions of the methods, as product package inserts may not be the same as the approved versions of the methods.
2 Incorporated by reference. See paragraph (c) of this section.
3 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and false-negative rate for total coliforms, using lactose broth, is less than 10 percent.
4 All filtration series must begin with membrane filtration equipment that has been sterilized by autoclaving. Exposure of filtration equipment to UV light is not adequate to ensure sterilization. Subsequent to the initial autoclaving, exposure of the filtration equipment to UV light may be used to sanitize the funnels between filtrations within a filtration series. Alternatively, membrane filtration equipment that is pre-sterilized by the manufacturer (i.e., disposable funnel units) may be used.
5 Multiple-tube and multi-well enumerative formats for this method are approved for use in presence-absence determination under this regulation.
6 Colisure® results may be read after an incubation time of 24 hours.
7 A multiple tube enumerative format, as described in Standard Methods for the Examination of Water and Wastewater 9221, is approved for this method for use in presence-absence determination under this regulation.
8 The following changes must be made to the EC broth with MUG (EC-MUG) formulation: Potassium dihydrogen phosphate, KH2PO4, must be 1.5g, and 4-methylumbelliferyl-Beta-D-glucuronide must be 0.05 g.
40 C.F.R. §141.852